[Expression of Notch receptors, ligands and downstream target genes in epidermis of hypertrophic scar]

Zhonghua Zheng Xing Wai Ke Za Zhi. 2009 Jan; 25(1): 41-5Xia W, Pan BH, Liu B, Zhang X, Ma FC, Wang YM, Yang XT, Liu D, Guo SZOBJECTIVE: To study the expression of Notch receptors, ligands and downstream target genes in hypertrophic scar and normal skin, and to investigate its role in the development of hypertrophic scar. METHODS: By immunohistochemistry, the expression of epidermal differentiation markers- beta1 integrin, keratin 14 (K14) and keratin 19 (K19), as well as Notch 1-4 and Jagged1 were examined in hypertrophic scars and normal skins. The expression of Notch downstream genes- P21 and P63 was analyzed with real-time quantitative PCR and immunohistochemistry staining. RESULTS: Histological analysis revealed a significant epidermal thickening in the hypertrophic scars, with excessive cell layers above the basal layer. Compared to the normal epidermis, the expression of beta1 integrin, K19 and K14 decreased in hypertrophic scars (P

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