The phenotype and potential origin of nestin cardiac myocyte-like cells following infarction.
Nestin((+)) cardiac myocyte-like cells were detected in the peri-infarct/infarct region of the ischemically damaged heart. The present study was undertaken to elucidate the phenotype and potential origin of nestin((+)) cardiac myocyte-like cells and identify stimuli implicated in their appearance.
In the infarcted human and rat heart, nestin((+)) cardiac myocyte-like cells were morphologically and structurally immature, exhibited a desmin-immunoreactive striated phenotype, expressed the beta1-adrenergic receptor and associated with an aberrant pattern of connexin-43 expression and/or organization.
Nestin((+)) cardiac myocyte-like cells were detected 24 hrs post ischemic injury and persisted in the infarcted rat heart for 9 months. In the normal rat heart, cardiac progenitor transcriptional factors Nkx2.5/GATA4 were detected in a subpopulation of nestin((+)) neural stem cells.
Following an ischemic insult, nestin((+))/Nkx2.5((+)) neural stem cells migrated to the peri-infarct/infarct region and appeared to be in a primordial state of differentiation to a nestin((+)) cardiac myocyte-like cell. The exposure of adult male rats to normobaric hypoxia (12% O2) for ten days failed to promote the appearance of nestin((+)) cardiac myocyte-like cells. Following osmotic pump delivery of isoproterenol to normal adult rats, nestin((+)) cardiac myocyte-like cells were detected, albeit the response was modest and secondary to tissue loss.
Thus, ischemia-induced appearance of nestin((+)) cardiac myocyte-like cells apparently represents an adaptive response to heal the infarcted heart. Nkx2.5/GATA4 expression in a subpopulation of resident neural stem cells provides the appropriate phenotype for their potential differentiation to a nestin((+)) cardiac myocyte-like cell.
"The phenotype and potential origin of nestin(+) cardiac myocyte-like cells following infarction".
J Appl Physiol. 2009 Aug 13; Beguin PC, El-Helou V, Assimakopoulos J, Clement R, Gosselin H, Brugada R, Villeneuve L, Rohlicek CV, Del Duca D, Lapointe N, Rouleau JL, Calderone A (Hubmed.org)
Related:
Cardiac Myocyte-Specific Expression of Inducible Nitric Oxide
The cannabinoid receptor type 2 promotes cardiac myocyte
Cardiac Myocyte-Specific Expression of Inducible Nitric Oxide
Reversal of cardiac myocyte dysfunction as a unique mechanism
Redox Sensing and Cardiac Myocyte Loss: Some Recent Insights
Left Ventricular Hypertrophy, Cardiac Myocyte Adaptation
NADPH oxidase signaling and cardiac myocyte function
Innate defense mechanism against virus infection within the ...
IK1 Heterogeneity Affects Genesis and Stability of Spiral Waves
Axonal guidance for sympathetic neuron to cardiac myocyte
Increased Cardiac Myocyte Progenitors in Failing Human Hearts
In the infarcted human and rat heart, nestin((+)) cardiac myocyte-like cells were morphologically and structurally immature, exhibited a desmin-immunoreactive striated phenotype, expressed the beta1-adrenergic receptor and associated with an aberrant pattern of connexin-43 expression and/or organization.
Nestin((+)) cardiac myocyte-like cells were detected 24 hrs post ischemic injury and persisted in the infarcted rat heart for 9 months. In the normal rat heart, cardiac progenitor transcriptional factors Nkx2.5/GATA4 were detected in a subpopulation of nestin((+)) neural stem cells.
Following an ischemic insult, nestin((+))/Nkx2.5((+)) neural stem cells migrated to the peri-infarct/infarct region and appeared to be in a primordial state of differentiation to a nestin((+)) cardiac myocyte-like cell. The exposure of adult male rats to normobaric hypoxia (12% O2) for ten days failed to promote the appearance of nestin((+)) cardiac myocyte-like cells. Following osmotic pump delivery of isoproterenol to normal adult rats, nestin((+)) cardiac myocyte-like cells were detected, albeit the response was modest and secondary to tissue loss.
Thus, ischemia-induced appearance of nestin((+)) cardiac myocyte-like cells apparently represents an adaptive response to heal the infarcted heart. Nkx2.5/GATA4 expression in a subpopulation of resident neural stem cells provides the appropriate phenotype for their potential differentiation to a nestin((+)) cardiac myocyte-like cell.
"The phenotype and potential origin of nestin(+) cardiac myocyte-like cells following infarction".
J Appl Physiol. 2009 Aug 13; Beguin PC, El-Helou V, Assimakopoulos J, Clement R, Gosselin H, Brugada R, Villeneuve L, Rohlicek CV, Del Duca D, Lapointe N, Rouleau JL, Calderone A (Hubmed.org)
Related:
Cardiac Myocyte-Specific Expression of Inducible Nitric Oxide
The cannabinoid receptor type 2 promotes cardiac myocyte
Cardiac Myocyte-Specific Expression of Inducible Nitric Oxide
Reversal of cardiac myocyte dysfunction as a unique mechanism
Redox Sensing and Cardiac Myocyte Loss: Some Recent Insights
Left Ventricular Hypertrophy, Cardiac Myocyte Adaptation
NADPH oxidase signaling and cardiac myocyte function
Innate defense mechanism against virus infection within the ...
IK1 Heterogeneity Affects Genesis and Stability of Spiral Waves
Axonal guidance for sympathetic neuron to cardiac myocyte
Increased Cardiac Myocyte Progenitors in Failing Human Hearts
