Wound healing in the jugal mucosa of rats with a cold blade scalpel and an ultrasonic harmonic scalpel.

Braz J Otorhinolaryngol. 2009 May-Jun; 75(3): 362-6Masi EC, Rocha SL, Mocellin M, Faria JLUltrasound harmonic scalpel has been recently introduced in otorhinolaryngological procedures. AIM: to assess macro and microscopic evolution of the healing process of wounds created in the jugal mucosa of rats by the use of ultrasound scalpel. METHOD: we used 30 Wistar rats in which we made mucosal incisions on the right jugal mucosa with the ultrasound harmonic scalpel (USHS) and on the left side with the cold blade scalpel (CBS). Macroscopic and microscopic evaluations were carried out on the third, seventh and fourteenth days of postoperative. For the microscopic evaluation we used HE to asses the inflammatory process and the Sirius Red approach for collagens type I and III. Anti-CD 3 antibodies and anti-factor VIII assessed the concentration of T-lymphocytes and neovessels. RESULTS: the USHS caused greater cell damage with reepitelization delay. Microscopy showed more intense inflammatory reactions and a loss in collagen build up, delay in scar maturation and a greater vessel neoformation. CONCLUSION: USHS brings about a greater lesion in the incision area; delayed regeneration; promotes greater inflammatory process and angiogenic activity; delays in fibroplasia and scar tissue maturation on the rats' jugal mucosa when compared to cold blade scalpel.

The spatial and temporal distribution of crop residue burning in the contiguous United States.

Sci Total Environ. 2009 Aug 1; McCarty JL, Korontzi S, Justice CO, Loboda TBurning crop residue before and/or after harvest is a common farming practice however; there is no baseline estimate for cropland burned area in the contiguous U.S. (CONUS). We present the results of a study, using five years of remotely sensed satellite data to map the location and areal extent of crop residue burning in the CONUS. Our burned area approach combines 500 m Moderate Resolution Imaging Spectroradiometer (MODIS) Normalized Burn Ratio (dNBR) data, with 1 km MODIS active fire counts calibrated using coincident high resolution satellite data to generate area estimates. Our results show that cropland burning is an extensive and recurring annual event in several states in the CONUS. On average, 1,239,000 ha of croplands burn annually, which is equivalent to 43% of the annual average area of wildland fires in the U.S., as reported by the United States Forest Service for the same period. Several states experience high levels (>30,000 ha yr(-1)) of crop residue burning, including Arkansas, California, Colorado, Florida, Idaho, Kansas, Louisiana, North Dakota, Oklahoma, Oregon, South Dakota, Texas, and Washington. Validation with high resolution burn scar imagery and GPS data collected during targeted field campaigns showed a moderate to high-level accuracy for our burned area estimates, ranging from 78 to 90%. Our approach provides a more consistent methodology for quantifying cropland burned area at regional scales than the previously available U.S. national and state-level statistics on crop residue burning.

Cytokine Expression in Human Cardiac

Cardiac myofibroblasts (CMF) play a key role in infarct repair and scar formation following myocardial infarction (MI) and are also an important source of proinflammatory cytokines. We postulated that interleukin-1 alpha (IL-1alpha), a potential early trigger of acute inflammation post-MI, could stimulate human CMF to express additional proinflammatory cytokines.

Further, we hypothesized that these effects may be modulated by the anti-inflammatory cytokine interleukin-10 (IL-10). Human CMF were cultured from atrial biopsies from multiple patients.

Interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and cardiotrophin-1 (CT-1) mRNA expression and secretion were measured using quantitative real-time RT-PCR and ELISA. IL-1alpha (0.001-10 ng/ml, 0-6 h) stimulated IL-1beta, TNFalpha and IL-6 mRNA expression with distinct temporal and concentration profiles, resulting in increased cytokine secretion.

The response to IL-1alpha was much greater than with TNFalpha. Neither IL-1alpha nor TNFalpha treatment modulated CT-1 mRNA expression. Immunoblotting with phospho-specific antibodies revealed that IL-1alpha stimulated the ERK-1/2, p38 MAPK, JNK, PI3K/Akt and NF-kappaB signaling pathways.

Pharmacological inhibitor studies indicated roles for PI3K/Akt and NF-kappaB pathways in mediating IL-1beta expression, and for NF-kappaB and p38 MAPK pathways in mediating TNFalpha expression. IL-1alpha-induced IL-6 mRNA expression was reduced by p38 MAPK inhibition, but increased by ERK and JNK pathway inhibitors. IL-10 produced a consistent but modest reduction in IL-1alpha-induced IL-6 mRNA levels (not IL-1beta or TNFalpha), but this was not reflected by reduced IL-6 protein secretion. In conclusion, IL-1alpha stimulates human CMF to express IL-1beta, TNFalpha and IL-6 via specific signaling pathways; responses that are unaffected by IL-10 exposure.

Key words: cardiac fibroblasts, inflammation, signal transduction, cytokines.

Interleukin-1{alpha} Stimulates Pro-inflammatory Cytokine Expression in Human Cardiac Myofibroblasts.
Am J Physiol Heart Circ Physiol. 2009 Jul 31; Turner NA, Das A, Warburton P, O'Regan DJ, Ball SG, Porter KE