Acne Scars

Acta Biomater. 2009 May 5; Wang T, Jiang XJ, Tang QZ, Li XY, Lin T, Wu DQ, Zhang XZ, Okello EBACKGROUND: Cellular transplantation represents a promising therapy for myocardial infarction (MI). However, it is limited by low transplanted cell retention and survival within the ischemic tissue. This study was designed to investigate whether injectable alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel could improve cell transplant retention and survival, reduce infarct expansion, and inhibit left ventricle (LV) remodeling. METHODS AND RESULTS: Bone marrow derived stem cells (BMSCs) were encapsulated in alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel and kept the morphologies during the cell culturing, MTT assays were used for in vitro cell viability studies of the hydrogel and showed non-cytotoxic. 7 days after MI, 100mul alpha-cyclodextrin solution containing 2o10(7) BMSCs and 100mul MPEG-PCL-MPEG solution were injected into the infarcted myocardium simultaneously, the solutions solidified immediately after injection. Injection of culture medium or cell alone served as controls. Four weeks after the treatments, histological analysis indicated that the hydrogel was absorbed, injection of BMSCs with hydrogel increased cell retention and vessel density around the infarct, and subsequently prevented scar expansion compared with BMSCs injection alone. Echocardiography studies showed that injection of BMSCs with hydrogel increased left ventricle ejection function, and attenuated left ventricular dilatation. CONCLUSION: This study indicated that injection of BMSCs with alpha-cyclodextrin/MPEG-PCL-MPEG hydrogel was an effective strategy which could enhance the effect of cellular transplantation therapy for myocardial infarction.